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Expression patterns were similar to those seen in rats, with NR5A1 expressed in both Sertoli and Leydig cells in a 1-year-old boy, but showing decreased expression in Sertoli cells in an 8-year-old boy.

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The authors noted that mouse Sf2 is alternatively spliced to produce 4 distinct transcripts. (2006) observed expression in both Leydig and Sertoli cells in the 7-day-old rat, but expression levels decreased in Sertoli cells by 21 days, and was present only in Leydig cells in the 56-day-old sexually mature rat.

In humans, quantitative RT-PCR and Western blot analysis of testicular tissue obtained from males at ages ranging from 1 year to 26 years showed increased expression with increasing age during testicular development.